Recombinant TRIM25 Monoclonal Antibody (E-AB-81492)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, Rat Rat Brain, C6, Hela Verified Samples in IHC: MCF-7 |
| Dilution | WB 1:1000-1:5000, IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | WB, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human TRIM25 |
| Abbre | TRIM25 |
| Synonyms | E3 ubiquitin/ISG15 ligase TRIM25, EFP, Estrogen responsive finger protein, Estrogen-responsive finger protein, RING finger protein 147, RNF 147, RNF147, TRI25, TRIM 25, Trim25, Tripartite m, Tripartite motif containing 25, Tripartite motif containing protein 25 |
| Swissprot | |
| Calculated MW | 71 kDa |
| Observed MW |
71 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction |
| Clone No. | R06-1H2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. |
Other Clones
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Other Formats
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Unconjugated
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