Recombinant Vimentin/VIM Monoclonal Antibody (AN300103P)

Name: Recombinant Vimentin/VIM Monoclonal Antibody
Brand: Elabscience
SKU: AN300103P
Price: 150.0 USD
Availability: InStock

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Recombinant Vimentin/VIM Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	20μL	$ 150.00	- +

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Verified Samples	Verified Samples in WB: C6, CHO-K1, HeLa, 3T3 in IHC: Human tonsil in IF: HeLa
Dilution	WB: 1:1000；IHC: 1:200-1:500；ICC/IF: 1:100；FC: 1:100
Isotype	IgG
Host	Rabbit
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, ICC/IF, FC
Clonality	Recombinant;Monoclonal
Immunogen	A synthetic peptide corresponding to the center region of the Human Vimentin/VIM
Abbre	VIM
Synonyms	HEL, Epididymis luminal protein, CTRCT, CTRCT30, HEL113, Vimentin, VIM, Epididymis luminal protein 113, VIME, FLJ36605, epididymis secretory sperm binding protein, CTRCT30, HEL113, vimentin, CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME, Vimentin
Swissprot	P08670
Calculated MW	54 kDa
Observed MW	54 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cytoplasm, Nucleus matrix, Cell membrane
Tissue Specificity	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Concentration	1 mg/mL
Buffer	0.2 μm filtered solution in PBS
Purification Method	Protein A
Research Areas	Neuroscience, Signal Transduction, Stem Cells, Cancer, Developmental Biology
Clone	A1217
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]