Recombinant VWF Monoclonal Antibody (AN300869L)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in IHC: Mouse lungs |
| Dilution | IHC 1:1000-1:4000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human VWF protein |
| Abbre | VWF |
| Synonyms | von Willebrand antigen, VWF, F8VWF, VWD, Coagulation factor VIII, Coagulation factor VIII VWF, Factor VIII related antigen, von Willebrand antigen 2, von Willebrand antigen II, Von Willebrand disease, Von Willebrand Factor |
| Swissprot | |
| Calculated MW | 309 kDa |
| Observed MW |
280 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Tissue Specificity | Plasma |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Cancer, Stem Cells |
| Clone No. | 9B6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. |
Other Clones
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Other Formats
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Unconjugated
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