RMDN2 Polyclonal Antibody (E-AB-52932)
For research use only.
| Verified Samples |
Verified Samples in WB: Human liver Verified Samples in IHC: Human liver cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human RMDN2 |
| Abbre | RMDN2 |
| Synonyms | member A1, BLOCK18, FAM82A, FLJ32954 , FLJ38143, Fam82a1, Family with sequence similarity 82, Family with sequence similarity 82 member A, MGC33318, Microtubule associated protein, PRO34163, PYST9371, Protein FAM82A1, Regulator of microtubu, hRMD 2, hRMD 4, hRMD-2, hRMD4 |
| Swissprot | |
| Calculated MW | 47 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane. Cytoplasm. Cytoplasm>cytoskeleton>spindle. Cytoplasm>cytoskeleton>spindle pole. In interphase localizes in the cytoplasm, and during mitosis localizes to the spindle microtubules and spindle poles. Also detected as large dots in the perinuclear region. |
| Concentration | 0.9 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The human protein FAM82A1, also known as regulator of microtubule dynamics 2 (RMD2), was one of three proteins identified as related to a family of microtubule-associated proteins in C. elegans. FAM82A1 contains multiple coiled-coil domains and localize to the spindle microtubules and spindle poles during cell division. During interphase, RMD2 localizes to the cytoplasm with protein observed in the microtubule lattice and perinuclear region. |
Other Clones
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Other Formats
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Unconjugated
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