SERPINA6 Polyclonal Antibody (D-AB-10351L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human plasma |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human SERPINA6 protein expressed by Mammalian |
| Abbre | SERPINA6 |
| Synonyms | CBG, Corticosteroid-binding globulin, SERPINA6, Serpin A6, Transcortin |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
60 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Immunology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Corticosteroid-binding globulin (CBG),also known as SerpinA6,is a non-inhibitory member of the serine proteinase inhibitor (serpin) superfamily. It is the high-affinity transport protein for glucocorticoids in vertebrate blood. CBG is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and CBG,and promotes a significant and local release of glucocorticoids (over 90% of them are bound to CBG in human plasma). In this context,CBG directs glucocorticoids to sites of inflammation,and plays in consequence a crucial role in efficient glucocorticoid action in physiology. The SerpinA6 protein is mainly secreted by the liver. This negative acute phase protein regulates free cortisol levels in the blood and distributes cortisol to its target tissues. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration,and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. There are three heritable,human CBG gene mutations that can reduce CBG-cortisol binding affinity and/or reduce circulating CBG levels. |
Other Clones
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Unconjugated
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