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For research use only.

Verified Samples Verified Samples in WB: Human bladder
Verified Samples in IHC: Human liver cancer, Human prostate cancer
Dilution WB 1:1000-1:5000,  IHC 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IHC
Clonality Polyclonal
Immunogen Fusion protein of human SLC30A6
Abbre SLC30A6
Synonyms FLJ31101,  MST103,  MSTP103,  SLC30A6,  Solute carrier family 30 (zinc transporter),  Solute carrier family 30 member 6,  ZNT6,  Zinc transporter 6,  Zinc transporter like 3,  ZnT-6,  ZnT6,  member 4,  member 6
Swissprot
Calculated MW 51 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Golgi apparatus>trans-Golgi network membrane. Found in vesicules.
Concentration 1.2 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Antigen affinity purification
Research Areas Cancer,  Metabolism,  Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A6, permit cellular efflux of zinc.
Other Clones

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Unconjugated

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