STAT1 Polyclonal Antibody (D-AB-10285L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse lung, Hela, A549 Verified Samples in IHC: Rat liver, Human spleen |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human STAT1 Protein expressed by E.coli |
| Abbre | STAT1 |
| Synonyms | CANDF7, DKFZp686B04100, IMD31A, IMD31B, IMD31C, ISGF 3, ISGF-3, OTTHUMP00000163552, OTTHUMP00000165046, OTTHUMP00000165047, OTTHUMP00000205845, Signal tr, Signal transducer and activator of transcription 1 91kD, Signal transducer and activator of transcription 1 91kDa |
| Swissprot | |
| Calculated MW | 87 kDa |
| Observed MW |
87 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors,Jak kinases (TYK2 and JAK1) are activated,leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize,associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor,that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes,which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma),STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF),migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes,inducing a cellular antiviral state. |
Other Clones
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Unconjugated
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