THY1 Polyclonal Antibody (E-AB-70323)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain, Mouse cortex, Mouse thymus, Rat brain, Rat cortex, Rat thymus |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein corresponding to Mouse CD90 |
| Abbre | THY1 |
| Synonyms | CD7, CD90, CD90 antigen, CDw90, FLJ33325, MGC128895, T, T25, THY1, Theta antigen, Thy 1, Thy 1 T cell antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein, Thy 1.2, Thy-1 antigen, Thy-1 membrane glycoprotein, Thy1, Thy1 T cell antigen, Thy1 antigen, Thy1.1, Thy1.2 |
| Swissprot | |
| Calculated MW | 18 kDa |
| Observed MW |
35-37 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Membrane. |
| Concentration | 320 μg/mL |
| Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Immunology, Neuroscience, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol(GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen.Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons.Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. |
Other Clones
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Other Formats
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Unconjugated
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