TRAF5 Polyclonal Antibody (D-AB-10305L)

For research use only.
Verified Samples |
Verified Samples in WB: Jurkat, Hela Verified Samples in IHC: Human lymph, Mouse kidney |
Dilution | WB 1:250-1:500, IHC 1:100-1:400 |
Isotype | IgG |
Host | Rabbit |
Reactivity | Human, Mouse |
Applications | WB, IHC |
Clonality | Polyclonal |
Immunogen | Recombinant Human TRAF5 RNF84 Protein expressed by E.coli |
Abbre | TRAF5 |
Synonyms | RING finger protein 84, RNF 84, RNF84, TNF receptor associated factor 5, TNF receptor-associated factor 5, TRAF 5, TRAF5, Traf5 |
Swissprot | |
Calculated MW | 64 kDa |
Observed MW |
64 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Antigen Affinity Purification |
Research Areas | Immunology |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain,a RING-type zinc finger,and two TRAF-type zinc fingers. TRAF proteins are associated with,and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Alternate transcriptional splice variants have been characterized. |
Other Clones
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Unconjugated
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