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Transglutaminases (TGs) Activity Colorimetric Assay Kit (E-BC-K840-M)

All Size Price Qty
96T $ 380.00
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For research use only.

Detection Principle Transglutaminases (TG) catalyze the transfer of acyl groups between glutamine residues and lysine residues or primary amines. TG-mediated protein crosslinking is essential for a variety of biological processes and diseases. TG is also widely used as a food processing aid in meat, fish, dairy and grain products.
The detection principle of this kit is: TG catalyzes the acyl transfer between glutamine residues and primary amines, the product forms a chromogenic substance with the chromogenic agent, and determines the activity of TG in the sample by measuring the absorbance change at 525 nm.
Detection Method Colorimetric method
Detection Instrument Microplate reader (520-530 nm, optimum wavelength: 525 nm)
Research Area Amino Acids And Proteins
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 3.0 U/L
Detection Range 3.0-25.0 U/L
Precision inter-assay CV: 5.0-6.1% | intra-assay CV: 2.0-2.9%
Sample Volume 120 μL
Assay Time 1 h 40 min
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