VCAM-1/CD106 Polyclonal Antibody(Capture/Detector) (AN000250P)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse spleen, Rat kidney, Rat spleen Verified Samples in IHC: Human Kidney cancer Verified Samples in ELISA: Recombinant Rat VCAM-1/CD106 protein, Rat serum, Rat plasma |
| Dilution | WB 1:500-1:1000, IHC 1:1000-1:2000, ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, ELISA Capture/Detector |
| Clonality | Polyclonal |
| Immunogen | Recombinant Rat VCAM-1/CD106 protein expressed by Mammalian |
| Abbre | VCAM-1/CD106 |
| Synonyms | CD106, Vcam1, Vcam-1, V-CAM 1, VascuLar ceLL adhesion protein 1, SoLubLe VascuLar CeLL Adhesion MoLecuLe-1 |
| Swissprot | |
| Calculated MW | 81 kDa |
| Observed MW |
75-100 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses,binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
Other Clones
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Unconjugated
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