Vitamin D-binding protein/DPB Polyclonal Antibody(Capture/Detector) (AN001150P)
For research use only.
| Verified Samples |
Verified Samples in ELISA: Recombinant Human Vitamin D-binding protein/DPB protein, Human serum, Human plasma Verified Samples in WB: Human plasma |
| Dilution | ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL, WB 1:1000-1:2000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | ELISA Capture/Detector, WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human Vitamin D-binding protein/DPB protein expressed by Mammalian |
| Abbre | Vitamin D-binding protein/DPB |
| Synonyms | Vitamin D-binding protein, gc-globulin, GC, DBP, DBP/GC, GRD3, VDBG, VDBP, |
| Swissprot | |
| Calculated MW | 53 kDa |
| Observed MW |
60 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Metabolism, Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition,it is also an actin scavenger,and is the precursor to the immunomodulatory protein,Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis,osteoclast development and possibly in macrophage activation/recruitment. |
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Unconjugated
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