For research use only.
| Verified Samples |
Verified Samples in WB: 293T, NIH/3T3, K562 |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human WDSUB1 |
| Abbre | WDSUB1 |
| Synonyms | SAM and U box domain containing protein 1, SAM and U-box domain containing 1, sterile alpha motif and U box domain containing 1 , 1700048E19Rik, 2610014F08Rik, FLJ36175, MGC108920, UBOX6 , WD repeat, WDSAM1 , WDSUB1 |
| Swissprot | |
| Calculated MW | 53 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 0.7 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | WDSUB1 (WD repeat, SAM and U-box domain-containing protein 1), also known as UBOX6 or WDSAM1, is a 476 amino acid protein that contains one SAM (sterile alpha motif) domain, one U-box domain and seven WD repeats.Existing as two isoforms due to alternative splicing, WDSUB1 is encoded by a gene located on chromosome 2.The second largest human chromosome, chromosome 2 encodes over 1,400 genes and comprises nearly 8% of the human genome, housing a number of disease-associated genes.Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes.Additionally, an extremely rare recessive genetic disorder, Alstr m syndrome, is caused by mutations in the ALMS1 gene, which maps to chromosome 2. |
Other Clones
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