E-Click EdU Cell Proliferation Assay Kits
The most accurate method to measure DNA proliferation is by directly measuring DNA synthesis. The most common method for this uses antibody-based detection of the nucleoside analog bromo-deoxyuridine (BrdU). EdU (5-ethynyl-2 '-deoxyuridine) is an alternative to BrdU, which is a thymidine nucleoside analogue that can be incorporated into replicating DNA molecules instead of thymidine (T) during cell proliferation. In contrast to BrdU assays, the EdU-Click assays are not antibody-based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. EdU utilizes click chemistry for detection in a variety of fluorescent dye readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.
Elabscience® provides EdU Cell Proliferation Assay Kits, including EdU Cell Proliferation Flow Cytometry Assay Kits and EdU Cell Proliferation Imaging Assay Kits, which do not need to use antibodies in detection, These kits offer convenient operation, high detection sensitivity, and represent a new method based on the BrdU method.
Features of Elabscience® E-Click EdU Cell Proliferation Assay Kits
Simplicity:
No antigen-antibody reaction is required, the process is simple and efficientSensitive:
Without using an antibody, the detection dye is only 1/500 of BrdU antibody, making it easy to spread and can be accurately detected even for a single proliferating cellFast:
No need to incubate overnight, only 2~3 hours are required to complete the testAccurate:
Since there is no need for DNA denaturation (acid hydrolysis, pyrolysis, enzymatic hydrolysis, etc.), this method effectively avoids sample damage caused by denaturationCompatibility:
Minimal damage to the sample, easier to simultaneously label with various antibodies or fluorescent proteins, and capable of detecting other cellular characteristics at the same timeEdU Cell Proliferation Detection Kits
| Product Name | Dye | Size | Price |
|---|---|---|---|
| E-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Green，FITC) | FITC | 50 Assays / 200 Assays | More Details |
| E-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Green, Elab Fluor® 488) | Elab Fluor® 488 | 50 Assays / 200 Assays | More Details |
| E-Click EdU Cell Proliferation Flow Cytometry Assay Kit (Red, Elab Fluor® 647) | Elab Fluor® 647 | 50 Assays / 200 Assays | More Details |
| E-Click EdU Cell Proliferation Imaging Assay Kit (Green，FITC) | FITC | 50 Assays / 200 Assays | More Details |
| E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) | Elab Fluor® 488 | 50 Assays / 200 Assays | More Details |
| E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) | Elab Fluor® 594 | 50 Assays / 200 Assays | More Details |
| E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 647) | Elab Fluor® 647 | 50 Assays / 200 Assays | More Details |
The Results of EdU
Detection result of
E-Click EdU Cell Proliferation Flow Cytometry Assay Kit
(Green, Elab Fluor® 488)(E-CK-A371)
Jurkat cells treated with 10 μM EdU for 4 h (red) and untreated with EdU for 4 h (blue)
Detection result of
E-Click EdU Cell Proliferation Imaging Assay Kit
(Green, Elab Fluor® 488) (E-CK-A376)
HeLa cells treated with 10 μM EdU for 2 h, proliferating cells (green), with DAPI staining (blue) for nuclei
