Uncoated ELISA Kits
Elabscience® Uncoated ELISA Kit contains the basic components used in ELISA experiments. For customers who have extensive experience with ELISA experiments and are able to establish their own ELISA systems, Uncoated ELISA Kits are the ideal cost-saving choice. This series of kits is a cost-effective alternative to pre-coated ELISA kits due to its ease of use and flexibility.
Features of Elabscience® Uncoated ELISA Kits
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Pretreatment and optimized
Matched antibody pairs and Micro ELISA pre-Plate are carefully screened and validated, greatly reducing the waste of time and money in the establishment and optimization of ELISA system.
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Flexible packaging
Flexible packaging of various specifications to meet the testing requirements of different sample quantities.
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Multiple targets
Over 700 Uncoated ELISA Kits extensively cover different targets in a variety of biological processes and sample types.
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Multiple detection methods
Kit provides the Capture & Detection Antibody and HRP Conjugate. In addition to complete colorimetric ELISA with Micro ELISA pre-Plate, chemiluminescence CLIA or other methods can be tried.
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Cost-effective
Cost-effective, for those who want to flexibly adjust coating concentration and match their own buffer solution to save costs, and explore detection system of special sample types, is the best choice.
Kit Contents
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Capture Antibody -
Reference Standard -
Micro ELISA pre-Plate -
Biotinylated Detection Antibody -
HRP Conjugate
Product Performance Display
Accuracy
Figure 1. Assessment of native and recombinant Mouse IL-10 using an uncoated ELISA kit
Stability
Figure 2. Stability assessment at 0, 4, 7, and 11 days at 37°C using a single kit lot
Precision
Table 1. Intra assay and inter assay precision verification of Uncoated Mouse IL-6 ELISA Kit
| Intra Assay Precision | Inter Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 93.02 | 175.23 | 762.56 | 95.55 | 165.32 | 690.87 |
| Standard deviation | 5.96 | 8.44 | 40.61 | 7.25 | 12.65 | 48.32 |
| CV (%) | 6.41 | 4.82 | 5.33 | 7.59 | 7.65 | 6.99 |
Inter-batch Consistency
Figure 3. Human IFN-γ quality control: high and low concentration samples (left) and corresponding standard curves (right) measured from 2022 to 2025
Recovery
Table 2. Recovery analysis of the Uncoated Human IL-6 ELISA Kit
| Sample Type | Range % | Average Recovery % |
|---|---|---|
| Serum (n=8) | 88-101 | 93 |
| EDTA Plasma (n=8) | 93-103 | 98 |
| Cell Culture Media (n=8) | 92-104 | 98 |
Hot Selling Uncoated ELISA Kits
Ancillary Reagent Kit
Elabscience® offers a 5-plate Ancillary Reagent Kit that contains all the single component reagent needed for the experiment :ELISA Plate Coating Buffer(5×), ELISA Plate Blocking Buffer, Sample Diluent, Biotinylated Antibody Diluent, HRP-conjugate Diluent, Wash Buffer for Sandwich-ELISA(25×), One-component TMB Substrate, Stop Solution(5×), Plate Sealer . Please refer to the instructions for a list of reagents needed
| Cat. No. | Product Name | Size |
|---|---|---|
| E-ELIR-K001 | ELISA Ancillary Reagent Kit | 96 Tx5 |
If you need to purchase single component (ancillary) reagents View Supplemental Products
