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The BCA concentration measured a protein concentration of 3mg/ml, but after adding buffer, no protein bands were found. What is the reason for this?

Source: Elabscience® Published: Sep 09,2024 Updated: Sep 09,2024

Suggest conducting comparative experiments, setting BSA as the positive control, adding buffer solution to the protein sample lysis buffer, mixing well, boiling and denaturing, and observing the band situation by electrophoresis on the sample.