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How many films can I make with 20μl of antibody with IHC experimental application?

Source: Elabscience®Published: Jul 01,2024

First of all, customers need to estimate the dosage of antibody according to the antibody instructions & the results of pre-experiments. Different samples will have different antibody dilution ratios due to different antigen expression. For example, in the IHC test of an antibody, the pre-test confirmation is calculated according to the dilution ratio of 1: 50. Under normal circumstances, one slice needs to add 100μl of diluted antibody, that is, one slice needs 2μl of antibody, and 20μl can make about 10 slices.