In IHC experiment, what are the conditions for antigen retrieval? Should I use EDTA or potassium citrate?

The repair conditions are generally high temperature and high pressure or microwave heating repair. For example: Antigen retrieval (high pressure method): Add 10 mmol/ml citrate buffer (pH 6.0) sufficient to submerge the slices in the pressure cooker (preparation: dissolve the antigen retrieval solution in the corresponding volume of ddH2O, mix well), and heat When it boils, place the slices on a heat-resistant plastic slicing rack, put them into the pot, cover the lid, fasten the pressure valve, continue heating, set the holding pressure for 4 minutes, open the vent valve to deflate after the time is up, and the pressure returns to zero. Open the lid, take out the inner pot and let it cool to room temperature. After the solution has cooled to room temperature, take out the slices (about 30 minutes). Antigen retrieval (microwave method): Add 10 mmol/ml citrate buffer (pH 6.0) sufficient to submerge the slices in a beaker (preparation: dissolve the antigen retrieval solution in the corresponding volume of ddH2O, mix well), and heat to boiling , place the slices on a heat-resistant plastic slice rack, put them into a beaker, heat over medium-low heat for 30 minutes, take them out, leave them to cool at room temperature, and take out the slices after the solution cools to room temperature (about 30 minutes). For the selection of IHC repair solution, it is recommended to refer to the antibody instruction manual, or refer to the following scheme: for human samples, it is preferred to use EDTA repair solution with pH 9.0 (clinical samples); for rat/mouse samples, it is preferred to use citric acid with pH 6.0 Repair fluid (scientific research sample).