Antibody & Protein
In case, cell lysis buffer could not completely lyse the cells: it was then vortexed after adding the RIPA to the sample cells, allowed to incubate on ice for 20 minutes, and centrifuge. Why is the cell lysate still viscous?
Source: Elabscience®Published: Jul 01,2024
After RIPA lysis buffer lyses the cells, the DNA in the nucleus diffuses into the sample relatively completely, forming a sticky liquid. In this case, it can be sonicated after lysis at 35~40% power using ice bath for a total of 1 min, sonicate for 2 s, and keep the interval 2 s to ensure that the cells were fully lysed and to reduce the viscosity of the sample.