Antibody & Protein
Is it okay to lyse cells with cell lysis buffer and then perform co-immunoprecipitation?
Source: Elabscience®Published: Jul 01,2024
No, the cell lysis buffer for co-immunoprecipitation is a lysis buffer that lyses cells under non-denaturing conditions. Conventional RIPA lysis contains SDS, which will denature the protein and affect the interaction between proteins. It is recommended to use denaturant-free lysis buffer for co-immunoprecipitation.