How to solve the problem of sample electrophoresis after denaturing with buffer solution, if the sample cannot run smoothly when loaded with a large amount of sample, and runs quickly and irregularly when loaded with a small amount of sample?

If the protein band movement rate is uneven, it is recommended to completely dissolve the loading buffer in a bath below 37 ℃ before adding the protein sample. Mix it evenly with the protein sample and boil for 10 minutes to completely denature the protein sample. If the protein band moves slowly, it is necessary to promptly check whether there is any leakage in the electrophoresis chamber.