The sample buffer needs to be thoroughly mixed before use, and the sample and sample buffer also need to be thoroughly mixed. Boiling and denaturing after thorough mixing can avoid such situations.
Antibody & Protein
After adding the sample buffer to the protein sample, boiling at 95 ° C for 10 minutes, and centrifuging at 14000, some samples may experience layering before being loaded. What is the reason for this?
Source: Elabscience® Published: Sep 27,2016 Updated: Sep 27,2016
