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The protein sample turns blue after boiling in buffer solution, but turns blue purple after being left at -80 ℃. What is the reason for this? Does it affect subsequent electrophoresis?

Source: Elabscience®Published: Aug 14,2024

Bromophenol blue will change from yellow to blue purple with the pH value, which does not affect the normal use of the product. Similar discoloration does not affect subsequent electrophoresis, and the experiment can continue.