Toll-free:1-888-852-8623

All categories

  • All categories
  • Flow Cytometry Antibodies
  • ELISA Kits
  • MACS Cell Isolation
  • Antibodies and Reagents
  • Cell Health Detection
  • Metabolism Assays
  • Immunoassays
  • Cell Identification
  • Proteins and Peptides
  • Cell Culture
Please enter the item number/product keyword!
Keyword cannot be empty !
INSERT SYMBOLS:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • UP ↑

What solution should be used to dissolve proteinase K during use? What is the commonly used working concentration? At what temperature should it be saved?

Source: Elabscience®Published: Aug 14,2024

The effective pH range of proteinase K is pH 4.0-12.5, and the optimal pH range is pH 7.5-8.0. Please use a buffer solution with the appropriate pH mentioned above, such as 10mM Tris HCl pH 7.4-7.8, to prepare the proteinase K solution according to the experimental requirements. The commonly used working concentration of proteinase K is 10-100ug/ml, and the storage solution concentration is generally 100-200mg/ml. It should be stored at -20 ℃ and diluted to the working concentration before use to avoid prolonged storage.