Why should I take the HRP-DAB TUNEL assay with shading light?

1. To avoid the effect of light on TdT enzyme activity. 2. Biotin molecules are easily decomposed by light, and the double bonds in biotin molecules are easily excited by light, and the decomposition produces substances such as ultraviolet light and active free radicals. These decomposition products will destroy the chemical binding between biotin and labeled molecules, thus affecting the accuracy and stability of experimental results.