Is it avaliable to take TUNEL and IF assays together?

It is avaliable therometically, but it is recommanded to take a pre-test as there is no such data yet. You might need to notice some key points: 1. Protease K for section sample treatment as permeability agent may interfere the antigen of immunofluorescence; 2. High temperature or high pressure for antigen repair in immunofluorescence assay may lead to DNA fragmentation, that will end up with false positive TUNEL results.