Can Annexin V kit be used on adherent cells?

Annexin V kit can be used on adherent cells, with the following caveat: 1. In late stage of apoptosis, cells may not able wo maintain adhesion, so floating cells should be collected for the test. 2. The dissociation of adherent cells should be handled gently to avoid cell damage caused by human operation. 3. Do not digest for too long with trypsin, avoid the usage of the trypsin contain EDTA. If EDTA-containing trypsin must be used, wash the cells to remove EDTA as much as possible.