What is the recommended storage condition (recommended minimum volume, buffer dissolution and concentration required and duration of solution storage) of calcein AM kit? Can the reagent be diluted to the used buffer concentration before being repackaged?

It is not recommended to dilute the dye before repackage, since it may lead to hydrolysis of Calcein AM as Calcein AM is more stable in stock solution. Calcein AM stock solution can be divided according to needs, and can be stored at -80°C for a longer shelf life. It is better to prepare the diluted buffer right before use, and use it up within one day.