Cell Function
Cell Function FAQs
In order to provide better customer support and answer customers' questions about cell function assay products, we have compiled and published answers to the frequently-asked questions (FAQs) about cell function assay products, and will continue to update them.
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No, it doesn't. What's more, antigen repair may cause DNA fragmentation and lead to false positive signal.
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Do not dry frozen section. Frozen section can be stored in -20°C within a week or in -80°C for longer time after being prepared. Before the experiment, rewarm the section at room temperature for about 20 minutes, fix at room temperature for 30-60 minutes with 4% of paraformaldehyde formaldehyde, then wash it and follow the procedure on the manual.
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The TdT equilibration buffer in the TUNEL kit is designed to provide optimal reaction conditions for the TdT enzyme.
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It is recommended to dilute with ultra-pure water, DO NOT use DEPC water. DEPC water is used to protect RNA or DNA from enzymatic hydrolysis, it will deactive Dnase I. DEPC water can be used to dissolve RNA when extracting RNA.
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Yes, soak in PBS and store at 4℃.
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If it has been fixed, it is not necessary to incubate and fix again, and subsequent experimental steps can be directly carried out.
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DO NOT use neutral balsam. The section is in a water-soluble state after dyeing, while neutral balsam is a water-insoluble agent that will blurred the sample. It is recommended to use a water-soluble sealer.
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Yes, it is. If DAPI is already contained in the anti-fluorescence quenching agent, there is no need to stain the sample with extra DAPI in the kit.
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No. TUNEL works by attaching a fluorescently labeled dUTP to the 3'-OH terminal of the fragmented DNA, it can only be worked once since there is no 3'-OH terminal after the operation.
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Cells with high proliferative or metabolic active may have a break in the DNA strand, both cases may end up with false-positive result. Evaluation of cell morphology may be used if there are doubts about the interpretation of the results.
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The tissue is transparent, and no water drop can be left on the slide.
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The main difference lies in the detection methods, for TUNEL in-situ kits, a fluorescence microscope is used for observing the results, as for TUNEL flow ytometry kit, the sample will be detected by a flow cytometer.
On the other hand, TUNEL in-situ kit is more suitable for tissue sections and cell slides or smears, while TUNEL flow ytometry kit is more suitable for suspended cells and adherent cells.
