JC-1 needs to detect the changes of fluorescence signal in both Ex/Em = 514/529 and Ex/Em = 585/590 at the same time to determine the changes of mitochondrial membrane potential, and there is fluorescence interference with GFP, so this kit cannot be applied to cells with GFP.
Cell Function
Is it able to use E-CK-A301 for cells with GFP?
Source: Elabscience®Published: Mar 04,2024
