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What is the difference between BrdU and EdU proliferation assay?

Source: Elabscience®Published: Mar 04,2024

BrdU method has many steps and requires the use of BrdU antibody, which has many influencing factors and poor stability. Based on EdU incorporation and subsequent click reaction, EdU method does not need to use antibodies, it has convenient operation and high detection sensitivity. It is a new method based on BrdU method, which will gradually replace BrdU method. EdU (5-ethynyl-2-deoxyuridine) is a thymidine analogue that can be incorporated into the newly synthesized DNA instead of thymidine in the process of DNA synthesis. The acetylene group on EdU can covalently react with fluorescence-labeled small molecular azide probes (such as FITC Azide, ElabFluor ®488 Azide, ElabFluor ®594 Azide, ElabFluor ®647 Azide) to form a stable triazole ring catalyzed by univalent copper ions, this reaction is called click reaction (Click reaction).