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To detect SOD in cell samples, is it feasible to treat cells with RIPA lysis buffer?

Source: Elabscience®Published: Mar 04,2024

SDS in RIPA lysis buffer interferes with enzyme activity determination, so it is not recommended to use RIPA lysis buffer for cell treatment. The SOD kit requires that the sample should not contain SDS, Tween20, NP-40, Triton X-100 and other detergents, and should not contain DTT, 2-mercaptoethanol and other reducing reagents.