The cultured cells are of the GC-1 spg cell line (mouse spermatogonial cells). Referring to Appendix Tables 2 and 3 of the manual, the incubation concentration and time are determined to be 50 μM and 2 hours, respectively. Under these conditions, will the 50 Assays kit be sufficient for 20 samples?

The 50 Assays kit contains 200 μL of EdU (10 mM), which can be prepared to a concentration of 50 μM in 40 mL. If using a 6-well plate with 2 mL per well, it would be exactly enough for 20 samples. It is recommended to use a 24-well plate with 500 μL per well, as the concentration and incubation time need to be confirmed through preliminary experiments, which will consume some reagents.

When preparing cell climbing slides, what size slides should be used for a 24‑well plate? 1 × 1 cm?

For a 24‑well plate, round coverslips with a diameter of 14 mm or square coverslips measuring 10 mm × 10 mm are typically used. The commonly used glass slide size is 75 mm × 25 mm; its width matches the edge of the plate for easy handling, and its length is sufficient to accommodate multiple coverslips for batch staining or observation.

Does this DMF evaporate?

Yes, DMF is quite volatile, so after use, make sure to tighten the cap and seal it with parafilm..

Cell Function

Cell Function FAQs

In order to provide better customer support and answer customers' questions about cell function assay products, we have compiled and published answers to the frequently-asked questions (FAQs) about cell function assay products, and will continue to update them.

The 50 Assays kit contains 200 μL of EdU (10 mM), which can be prepared to a concentration of 50 μM in 40 mL. If using a 6-well plate with 2 mL per well, it would be exactly enough for 20 samples. It is recommended to use a 24-well plate with 500 μL per well, as the concentration and incubation time need to be confirmed through preliminary experiments, which will consume some reagents.
For a 24‑well plate, round coverslips with a diameter of 14 mm or square coverslips measuring 10 mm × 10 mm are typically used. The commonly used glass slide size is 75 mm × 25 mm; its width matches the edge of the plate for easy handling, and its length is sufficient to accommodate multiple coverslips for batch staining or observation.
Yes, DMF is quite volatile, so after use, make sure to tighten the cap and seal it with parafilm..

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Cell Function

Cell Function

Cell Function FAQs

Q2. When preparing cell climbing slides, what size slides should be used for a 24‑well plate? 1 × 1 cm?

Q3. Does this DMF evaporate?