ELISA Research
Sample Dilution method
Source: Elabscience®Published: Mar 05,2024
If your test sample needs dilution, refer to the dilution method as follows:
For 100 fold dilution: One-step dilution. Add 5 μL sample to 495 μL sample diluent toyield 100 fold dilution.
For 1000 fold dilution: Two-step dilution. Add 5 μL sample to 95 μL sample diluent toyield 20 fold dilution, then add 5 μL 20 fold diluted sample to 245 μL sample diluent,after this, the neat sample has been diluted at 1000 fold successfully.
For 100000 fold dilution: Three-step dilution. Add 5 μL sample to 195 μL sample diluentto yield 40 fold dilution, then add 5 μL 40 fold diluted sample to 245 μL sample diluentto yield 50 fold dilution, and finally add 5 μL 2000 fold diluted sample to 245 μL samplediluent, after this, the neat sample has been diluted at 100000 fold successfully.
The amount of liquid taken at each dilution step is not less than 3μL, and the dilution ratio is not more than 100 times. Each dilution should be mixed evenly to avoid bubbles