ELISA Research FAQs

Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.

After the collection with the swab, elute with 500μl PBS, centrifuge at 5000×g for 10 minutes (if the sample remains turbid, try centrifuging at 10000×g), then collect the supernatant for testing.

Currently, our ELISA kits are only suitable for animal samples, and we do not have kits for testing plant/microbial samples.

No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.

Our ELISA kits have not been validated for compatibility with automated analyzers. The provided instructions are for manual operation. Customers may validate them on their own if needed.

To ensure temperature fluctuations during incubation stay within 1°C, it's recommended to use an incubator. If an incubator is not available, a water bath may be used, but precautions should be taken to avoid contaminating the plate with water. Using a shaker, especially one with vibration, for incubation is not recommended.

Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.

At present, hamster samples have not been verified for detection in mouse kits, and it is not a same species, so testing is not recommended.

It can be used, but cell culture requires a sterile environment, ELISA does not need to be sterile, we recommend that customers try not to use this instrument, to avoid doing other experiments such as cell culture caused by pollution, you can choose a conventional oven or water bath

According to the different bottom, it is divided into flat bottom, U-shaped bottom, V-shaped bottom and so on. The index of refraction of the flat bottom is low, which is suitable for detection in the enzyme reader. According to the color can be divided into transparent, black, white. Transparent is commonly used for the most general enzyme-linked immunoassay.

The sample does not need to dilute into a uniform concentration and then the sample is loaded. The tissue sample can be made into 10% tissue homogenate, and then the total concentration is detected by BCA method, and then the ELISA test is performed. The concentration of target protein detected by ELISA/the concentration of total tissue protein can be obtained in the tissue sample (pg/mg prot).