|
Purpose |
Sample |
Antibody Collocation |
|
|
Adjust the voltage |
1 |
Blank |
|
|
Adjust compensation |
2 |
CD45-Elab Fluor® Red 780 |
|
|
3 |
CD3-FITC、CD19-FITC、CD56-FITC、CD14-FITC |
||
|
4 |
HLA-DR-Elab Fluor® Violet 450 |
||
|
5 |
CD123-PE |
||
|
6 |
CD11c-APC |
||
|
FITC-FMO in combination with Isotype Control for auxiliary gating |
7 |
CD45-Elab Fluor® Red 780、HLA-DR-Elab Fluor® Violet 450、CD123-PE、CD11c-APC、Mouse IgG2a, κ Isotype Control-FITC、Mouse IgG1, κ Isotype Control-FITC |
|
|
Elab Fluor® Violet 450-FMO in combination with Isotype Control for auxiliary gating |
8 |
CD45-Elab Fluor® Red 780、CD3-FITC、CD19-FITC、CD56-FITC、CD14-FITC、CD123-PE、CD11c-APC、Mouse IgG2a, κ Isotype Control-Elab Fluor® Violet 450 |
|
|
PE-FMO in combination with Isotype Control for auxiliary gating |
7 |
CD45-Elab Fluor® Red 780、CD3-FITC、CD19-FITC、CD56-FITC、CD14-FITC、HLA-DR-Elab Fluor® Violet 450、CD11c-APC、Mouse IgG1, κ Isotype Control-PE |
|
|
APC-FMO in combination with Isotype Control for auxiliary gating |
8 |
CD45-Elab Fluor® Red 780、CD3-FITC、CD19-FITC、CD56-FITC、CD14-FITC、HLA-DR-Elab Fluor® Violet 450、CD123-PE、Mouse IgG1, κ Isotype Control-APC |
|
|
Full panel |
9 |
CD45-Elab Fluor® Red 780、CD3-FITC、CD19-FITC、CD56-FITC、CD14-FITC、HLA-DR-Elab Fluor® Violet 450、CD123-PE、CD11c-APC |
|
Marker |
Fluorochrome |
Clone No. |
Cat. No. |
|
CD45 |
Elab Fluor® Red 780 |
HI30 |
|
|
CD3 |
FITC |
OKT3 |
|
|
CD19 |
FITC |
CD19 |
|
|
CD56 |
FITC |
MY31 |
|
|
CD14 |
FITC |
M5E2 |
|
|
HLA-DR |
Elab Fluor® Violet 450 |
L243 |
|
|
CD123 |
PE |
6H6 |
|
|
CD11c |
APC |
BU15 |
|
|
Mouse IgG2a, κ Isotype Control |
FITC |
C1.18.4 |
|
|
Mouse IgG1, κ Isotype Control |
FITC |
MOPC-21 |
|
|
Mouse IgG2a, κ Isotype Control |
Elab Fluor® Violet 450 |
C1.18.4 |
|
|
Mouse IgG1, κ Isotype Control |
PE |
MOPC-21 |
|
|
Mouse IgG1, κ Isotype Control |
APC |
MOPC-21 |
%20in%20Human%20Peripheral%20Blood.webp)
Tips:
1. Dendritic cells (DCs) in human peripheral blood are classified into plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells (mDCs).
2. pDC: lin(CD3, CD19, CD56, CD14)-HLA-DR+CD123+CD11C-; mDC: lin(CD3, CD19, CD56, CD14)-HLA-DR+CD123-CD11c+.
3. Dendritic cells (DCs) account for a very low percentage in whole blood and are usually difficult to detect directly. Therefore, an alternative approach is to isolate peripheral blood mononuclear cells (PBMCs) first prior to detection.
4.The staining procedure for the FMO control, used in conjunction with the isotype control, is the same as that for the sample, except that the staining for one target marker is omitted and replaced with the corresponding isotype control. This control reveals the background fluorescence contributed by all reagents except for that specific one, thereby providing more accurate guidance for setting the gating boundaries.
