|
Purpose |
Sample |
Antibody Collocation |
|
|
Adjust the voltage |
1 |
Blank |
|
|
Adjust compensation |
2 |
CD11b-PE |
|
|
3 |
Ly-6G-FITC |
||
|
4 |
Gr-1-APC |
||
|
PE-FMO in combination with Isotype Control for auxiliary gating |
5 |
Ly-6G-FITC、Gr-1-APC、Rat IgG2b, κ Isotype Control-PE |
|
|
FITC-FMO in combination with Isotype Control for auxiliary gating |
6 |
CD11b-PE、Gr-1-APC、Rat IgG2a, κ Isotype Control-FITC |
|
|
APC-FMO in combination with Isotype Control for auxiliary gating |
7 |
CD11b-PE、Ly-6G-FITC、Rat IgG2b, κ Isotype Control-APC |
|
|
Full panel |
8 |
CD11b-PE、Ly-6G-FITC、Gr-1-APC |
|
Marker |
Fluorochrome |
Clone No. |
Cat. No. |
|
CD11b |
PE |
M1/70 |
|
|
Ly-6G |
FITC |
1A8 |
|
|
Gr-1 |
APC |
RB6-8C5 |
|
|
Rat IgG2b, κ Isotype Control |
PE |
LTF-2 |
|
|
Rat IgG2a, κ Isotype Control |
FITC |
2A3 |
|
|
Rat IgG2b, κ Isotype Control |
APC |
LTF-2 |
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Tips:
1. Murine MDSCs are divided into granulocytic MDSCs (g-MDSCs; CD11b⁺Gr-1⁺Ly6G⁺) and monocytic MDSCs (m-MDSCs; CD11b⁺Gr-1⁺Ly6G⁻).
2. The Gr-1 antibody recognizes both Ly6G and Ly6C. In this experiment, MDSCs are classified based on the expression profiles of Gr-1 and Ly6G.
3. In this experiment, CD11b-positive cells were gated to analyze the expression of Gr-1 and Ly6G. The Gr-1⁺Ly6G⁻ population was identified as monocytic MDSCs (M-MDSCs), while the Gr-1⁺Ly6G⁺ population was identified as granulocytic MDSCs (G-MDSCs).
