|
Purpose |
Sample |
Antibody Collocation |
|
|
Adjust the voltage |
1 |
Blank |
|
|
Adjust compensation |
2 |
CD11b-PE |
|
|
3 |
Ly-6G-FITC |
||
|
4 |
Ly-6C-APC |
||
|
PE-FMO in combination with Isotype Control for auxiliary gating |
5 |
Ly-6G-FITC、Ly-6C-APC、Rat IgG2b, κ Isotype Control-PE |
|
|
FITC-FMO in combination with Isotype Control for auxiliary gating |
6 |
CD11b-PE、Ly-6C-APC、Rat IgG2a, κ Isotype Control-FITC |
|
|
APC-FMO in combination with Isotype Control for auxiliary gating |
7 |
CD11b-PE、Ly-6G-FITC、Rat IgG2b, κ Isotype Control-APC |
|
|
Full panel |
8 |
CD11b-PE、Ly-6G-FITC、Ly-6C-APC |
|
Marker |
Fluorochrome |
Clone No. |
Cat. No. |
|
CD11b |
PE |
M1/70 |
|
|
Ly-6G |
FITC |
1A8 |
|
|
Ly-6C |
APC |
Monts 1 |
|
|
Rat IgG2b, κ Isotype Control |
PE |
LTF-2 |
|
|
Rat IgG2a, κ Isotype Control |
FITC |
2A3 |
|
|
Rat IgG2a, κ Isotype Control |
APC |
2A3 |
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Tips:
1. The granulocytic MDSC (G-MDSC) phenotype is defined as CD11b⁺Ly-6ClowLy-6G⁺, whereas the monocytic MDSC (M-MDSC) phenotype is CD11b⁺Ly-6ChighLy-6G⁻.
2. In this experiment, CD11b-positive cells were gated to analyze the expression of Ly-6C and Ly-6G. Granulocytic MDSCs (G-MDSCs) and monocytic MDSCs (M-MDSCs) were distinguished based on their characteristic high expression of Ly-6G and Ly-6C, respectively.
