Flow Cytometry
Detection of TB cells (4-color) in mouse lymph node
Source: Elabscience®Published: Dec 19,2023
|
Purpose |
Sample |
Antibody Collocation |
|
Adjust the voltage |
1 |
Blank |
|
Full panel |
2 |
CD3-Elab Fluor® Violet 450, CD4-PE, CD8a-FITC, CD19-APC |
|
Marker |
Fluorochrome |
Clone No. |
Cat. No. |
|
CD3 |
Elab Fluor® Violet 450 |
17A2 |
|
|
CD4 |
PE |
GK1.5 |
|
|
CD8a |
FITC |
53-6.7 |
|
|
CD19 |
APC |
1D3 |
Tips:
1. The lymph nodes are mainly composed of lymphocytes, so there is no need for CD45 Marker.
2. CD3/CD4/CD8/CD19 cells are easy distinguished, Single Positive, FMO and Isotype Control are unnecessary.
