Flow Cytometry
Detection of T/B/NK (3-color) in Rat Spleen
Source: Elabscience®Published: Dec 19,2023
|
Purpose |
Sample |
Antibody Collocation |
|
Adjust the voltage |
1 |
Blank |
|
Adjust compensation |
2 |
CD3-FITC |
|
3 |
CD45RA-PE |
|
|
4 |
CD161-APC |
|
|
APC-FMO in combination with Isotype Control for auxiliary gating |
5 |
CD3-FITC, CD45RA-PE; Mouse IgG1, κ Isotype Control-APC |
|
PE-FMO in combination with Isotype Control for auxiliary gating |
6 |
CD3-FITC, CD161-APC; Mouse IgG1, κ Isotype Control-PE |
|
Full panel |
7 |
CD3-FITC, CD45RA-PE, CD161-APC |
|
Marker |
Fluorochrome |
Clone No. |
Cat. No. |
|
CD3 |
FITC |
G4.18 |
|
|
CD45RA |
PE |
OX-33 |
|
|
CD161 |
APC |
3.2.3 |
|
|
Mouse IgG1, κ Isotype Control |
PE |
MOPC-21 |
|
|
Mouse IgG1, κ Isotype Control |
APC |
MOPC-21 |
Tips:
1. The phenotype of rat B cells is CD3-CD45RA+, and the phenotype of NK cells is CD3-CD161+.
2. Isotype Control for CD45RA and CD161 is suggested for auxiliary gating.
