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Detection of T cells (4-color) in Human Peripheral Blood

Source: Elabscience®Published: Dec 21,2023

Purpose

Sample

Antibody Collocation

Adjust the voltage

1

Blank

Full panel

2

CD45-Elab Fluor® Violet 450, CD3-APC, CD4-FITC, CD8a-PE

Marker

Fluorochrome

Clone No.

Cat. No.

CD45

Elab Fluor® Violet 450

HI30

E-AB-F1137Q

CD3

APC

OKT3

E-AB-F1001E

CD4

FITC

RPA-T4

E-AB-F1109C

CD8a

PE

OKT-8

E-AB-F1110D

Tips:

1. For human peripheral blood T cells, it is suggested to use CD45, which can easily gate the lymphocyte population.

2. The above panel shows clear cell populations, and there is no need to set single staining tubes for compensation.