Flow Cytometry
Detection of T cells (4-color) in Human Peripheral Blood
Source: Elabscience®Published: Dec 21,2023
|
Purpose |
Sample |
Antibody Collocation |
|
Adjust the voltage |
1 |
Blank |
|
Full panel |
2 |
CD45-Elab Fluor® Violet 450, CD3-APC, CD4-FITC, CD8a-PE |
|
Marker |
Fluorochrome |
Clone No. |
Cat. No. |
|
CD45 |
Elab Fluor® Violet 450 |
HI30 |
|
|
CD3 |
APC |
OKT3 |
|
|
CD4 |
FITC |
RPA-T4 |
|
|
CD8a |
PE |
OKT-8 |
Tips:
1. For human peripheral blood T cells, it is suggested to use CD45, which can easily gate the lymphocyte population.
2. The above panel shows clear cell populations, and there is no need to set single staining tubes for compensation.
