Detection of Lymphocyte (4-color) in Mouse Tumor

Source: Elabscience^®Published: Dec 21,2023

Purpose	Sample	Antibody Collocation
Adjust the voltage	1	Blank
Adjust compensation	2	CD45-PerCP/Cyanine5.5
	3	CD3-FITC
	4	CD4-PE
	5	CD8-APC
Full panel	6	CD45-PerCP/Cyanine5.5, CD3-FITC, CD4-PE, CD8a-APC

Marker	Fluorochrome	Clone No.	Cat. No.
CD45	PerCP/Cyanine5.5	30-F11	E-AB-F1136J
CD3	FITC	17A2	E-AB-F1013C
CD4	PE	GK1.5	E-AB-F1097D
CD8a	APC	53-6.7	E-AB-F1104E

Detection of Lymphocyte (4-color) in Mouse Tumor-1

Tips:

1. In tumor tissue, the majority cells are tumor cells, and the proportion of lymphocytes is relatively low. CD45 and SSC can be used to gate the lymphocytes.

2. The lymphocyte gate in tumor cells is defined as the gate of CD45^high and SSC^low on the CD45/SSC scatter plot.

3. This experimental protocol is applicable to the detection of lymphocytes in non-lymphoid or hematopoietic cancer samples.