Empowering Primary Immune Cell Research: High-Efficiency Human CD4⁺/CD8⁺ T Cell and B Cell Sorting

At the forefront of immunological research, whether exploring the precise killing and regulation of T cells or unraveling antibody secretion and antigen presentation by B cells, the foundation of experimentation consistently relies on obtaining primary immune cells of high purity, high viability, and undisturbed physiological function. While traditional positive selection methods can achieve high-purity cells, the binding of antibodies to key cell surface receptors (e.g., CD3, CD4, CD19) can readily alter the cells' native state, potentially impacting downstream functional assays, signaling studies, and even the development of cell-based therapies.

Addressing this critical challenge, the Elabscience^® R&D team, leveraging its well-established magnetic bead ‑ based negative selection technology platform, now introduces the Human CD4/CD8 T Cell and B Cell Negative Isolation Kits. Utilizing a negative isolation approach with magnetic beads, these kits eliminate the need for direct labeling of human CD4/CD8 T cells and B cells, thereby avoiding antibody‑induced cell activation or epitope masking. This preserves the cells in their native state, with all surface receptors and molecules (such as CD3/CD28/PD‑1/CD40/BCR, etc.) maintaining their intact, native conformation. These kits represent the golden starting point for functional activation studies, signaling pathway investigations, drug target validation, and cell therapy engineering.

Table of Contents

1. Introduction to Cell Isolation Products

2. Features of Cell Isolation Products

3. Experimental Validation Results of Cell Isolation Products

01 Introduction to Cell Isolation Products

The Elabscience^® Human CD4/CD8 T Cell and B Cell Negative Isolation Kit isolates CD4/CD8 T cells and B cells from fresh human peripheral blood PBMC samples via a negative selection method. Its principle involves labeling non-target cells (non-CD4/CD8 T cells and non-B cells) with different biotin‑conjugated monoclonal antibodies, followed by removal using streptavidin‑coated magnetic beads. This achieves the isolation of human peripheral blood PBMC‑derived CD4/CD8 T cells and B cells. It preserves the unstimulated, native state of the target cells, and the obtained cells are free of any antibody or bead labeling, making them directly suitable for downstream applications. This product series mainly includes the following contents:

Table 1. EasySort™ Human T and B Cell Isolation Kits

02 Features of Cell Isolation Products

No red blood cell lysis required: ensures better cell viability

No stimulation: target cells are free of antibody or bead labeling, preserving their natural state

High purity: achieves >95% purity post‑selection

No separation column needed: simplifies the workflow

Short processing time: negative selection can be completed in as little as 15 minutes

Broad sample compatibility: suitable for fresh or cryopreserved human PBMC samples

03 Experimental Validation Results of Cell Isolation Products

Fig. 1 The purity of CD4+ T cells before and after sorting was analyzed by flow cytometry using Elab Fluor^® Violet 450 Anti-Human CD3 Antibody [OKT-3] (E-AB-F1001Q) and APC Anti-Human CD4 Antibody [SK3] (E-AB-F1352E). The proportions of CD3⁺CD4⁺ T cells before and after sorting were 25.42% and 96.92%, respectively.

Fig. 2 The purity of CD8+ T cells before and after sorting was analyzed by flow cytometry using Elab Fluor^® Violet 450 Anti-Human CD3 Antibody [OKT-3] (E-AB-F1001Q) and PE Anti-Human CD8a Antibody [HIT8a] (E-AB-F1271D). The proportions of CD3⁺CD8⁺ T cells before and after sorting were 16.97% and 95.79%, respectively.

Fig. 3 The purity of human B cells was analyzed by flow cytometry using PerCP Anti-Human CD45 Antibody [HI30] (E-AB-F1137F), PE Anti-Human CD3 Antibody [OKT-3] (E-AB-F1001D), and APC Anti-Human CD19 Antibody [CB19] (E-AB-F1004E). The purity of CD45⁺CD3⁻CD19⁺ human B cells in normal human peripheral blood mononuclear cell samples before and after sorting was 4.92% and 93.64%, respectively.

Table 2. T Cell & B Cell Research Products

Table 3. Multi‑Color Flow Cytometry Antibodies for T & B Cells

The above provides an overview of the Elabscience^® Human CD4/CD8 T Cell and B Cell Negative Isolation Kit. In the future, Elabscience^® will also launch the Human NK Cell Negative Isolation Kit. If you have any questions or require detailed technical documents, please feel free to leave a comment or contact us directly!