Flow Cytometry
How to Choose the Right Red Blood Cell Lysis Buffer
Source: Elabscience®Published: Nov 14,2024
Choosing the Right Red Blood Cell Lysis Buffer for Accurate Blood Analysis
In blood samples, red blood cells (RBCs) can interfere with white blood cell studies. This video explains how to select the appropriate RBC lysis buffer for effective removal without damaging nucleated cells!
In this video, you'll learn:
● How RBC Lysis Works: Understand the principle of lysis based on osmotic pressure differences.
● Types of Lysis Buffers: Learn the differences between buffers with and without fixatives — and why fixative-free options are generally preferred.
● Safety and Special Cases: Discover when to use fixative-containing buffers, such as when handling samples with viruses.
● Best Practices: Get tips on dilution, timing, and preparing the lysis buffer for optimal results.
