Flow Cytometry
Impact of PMA Stimulation on CD4 Detection and Solutions
Source: Elabscience®Published: Nov 14,2024
Welcome to our Flow Cytometry Panel Design series - Impact of PMA Stimulation on CD4 Detection and Solutions.
In immunological research, accurate detection of T cell subsets, especially Th cells (CD4+ T cells), is crucial for understanding the immune system's functionality. However, PMA stimulation can significantly affect CD4 expression, complicating detection efforts.
In this video, we'll explore:
● The importance of Th cell detection and the challenges posed by low resting quantities and PMA stimulation.
● Key findings from Michael Bigby's 1990 study that revealed how PMA leads to CD4 internalization in various cell types.
● Effective strategies to mitigate PMA-induced CD4 downregulation, including using brighter fluorescent labels and calcium ion chelators like QUIN-2 AM.
● Introduction of Elabscience®'s Human CD4 (SK3) and Mouse CD4 (RM4-5) clones as innovative solutions for clearer CD4+ subset identification post-stimulation.
