Functions, Identification Methods, and Significance of Th17 Cells

In this session, we're exploring the fascinating world of Th17 cells, a unique subset of T helper cells with pivotal roles in immune regulation and disease processes.

Th17 cells are not only crucial for defending against pathogens but are also deeply involved in autoimmune diseases, inflammatory disorders, and tumor immunity. Their identification and analysis open doors to better understanding and treating complex diseases.

In this video, we’ll cover:

● What Th17 cells are, their primary functions, and their significance in immune responses.

● How naive CD4+ T cells differentiate into Th17 cells through antigen and cytokine stimulation.

● The signaling pathways that drive Th17 differentiation, including TGF-β, IL-6, IL-1, and STAT3 activation.

● Methods for identifying Th17 cells, focusing on flow cytometry and the detection of markers like CD4, CCR6, RORγt, and IL-17A.

● Best practices for cytokine detection, including the use of stimulation blockers and fixation-permeabilization techniques.

● Clinical significance of Th17 cells in conditions like rheumatoid arthritis, psoriasis, and tumor immunity.

By the end of this video, you'll gain insights into the biology and clinical relevance of Th17 cells and learn how to effectively study them to advance your research!