Mouse/Rat Monoclonal Antibody Service
Mouse/Rat monoclonal antibodies are immunoglobulins produced by hybridoma cells against a single epitope. Their greatest advantages are strong specificity, high sensitivity and strong batch stability. In recent years, monoclonal antibodies have shown important research value in protein research, therapeutics, and diagnostics.
Elabscience® strive to provide you mouse/rat monoclonal antibodies which could support your needs to its full extent. We have established monoclonal antibody platform with years of antibody R & D and production experience, the service processes of monoclonal antibody production, screening, purification and detection are upgraded with high success rate. We have a lot of experience in the field and our core technology is top-of-the-line. We offer comprehensive, one-stop comprehensive support for all phases of mouse/rat antibody development.
Service Highlights
Leading technology and platforms
20+ years Experience in antibody production
>95% High successful rate
A wide range of validation platforms ELISA, WB, IHC, IF, FACS, and more.
Individual service for customers
Service Flow
Immunogen preparation
Immunization
Myeloma cell culture
Hybridoma fusion and positive clones screening
Hybridoma culture and antibody purification
QC and subtype identification
Service Process
| Steps | Specification | Timeline | Deliverables | Pricing |
|---|---|---|---|---|
| Immunogen Preparation |
•Recombinant protein expression
•Peptide synthesis and conjugation
|
4 weeks |
• Design report
• Protein sample
• SDS-PAGE result
|
Inquiry |
| Immunization and Titer test |
•5-10 Balb/c immunization Anti-serum titer ELISA test
|
7-8 weeks |
• Anti-serum sample
• ELISA titer
|
|
| Fusion & Screening |
•Myeloma cell culture
•Hybridoma fusion
•Positive clones screening
•2 rounds of subcloning
|
4weeks |
• 10-30 ELISA positive clones
• 1-2 ml supernatant per clone
|
|
| Antibody production & purification |
•hybridoma culture
•Antibody purification
|
2 weeks |
• 3-5 mg antibodies per clone
|
|
| QC & subtype identification |
•SDS-PAGE
•antibody subtype verification
|
1-2 weeks |
• project report
|
Projects Presentation
Mouse anti-Zebra Fish VASA mAb published in Front Endocrinol (Lausanne). 2022 Feb 8;12:820463
Mouse anti-P. gingivalis mAb published in ONCOLOGY LETTERS 21: 189, 2021
Rat mAb anti low Density Lipoprotein Receptor published in Circulation. 2022;145:675–687
Mouse anti-SETD3 Mab published in Cell Death Dis. 2019;10(3):183.
