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Mouse/Rat Monoclonal Antibody Service

Mouse/Rat monoclonal antibodies are immunoglobulins produced by hybridoma cells against a single epitope. Their greatest advantages are strong specificity, high sensitivity and strong batch stability. In recent years, monoclonal antibodies have shown important research value in protein research, therapeutics, and diagnostics.

Elabscience® strive to provide you mouse/rat monoclonal antibodies which could support your needs to its full extent. We have established monoclonal antibody platform with years of antibody R & D and production experience, the service processes of monoclonal antibody production, screening, purification and detection are upgraded with high success rate. We have a lot of experience in the field and our core technology is top-of-the-line. We offer comprehensive, one-stop comprehensive support for all phases of mouse/rat antibody development.

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Stable Cell Line Development Services

Service Highlights

Leading technology and platforms

Leading technology and platforms

20+ years Experience in antibody production

20+ years Experience in antibody production

>95% High successful rate

>95% High successful rate

A wide range of validation platforms ELISA, WB, IHC, IF, FACS, and more.

A wide range of validation platforms ELISA, WB, IHC, IF, FACS, and more.

Individual service for customers

Individual service for customers

Service Flow

Immunogen preparation

Immunogen preparation

Immunization

Immunization

Myeloma cell culture

Myeloma cell culture

Hybridoma fusion and positive clones screening

Hybridoma fusion and positive clones screening

Hybridoma culture and antibody purification

Hybridoma culture and antibody purification

QC and subtype identification

QC and subtype identification

Service Process

Steps Specification Timeline Deliverables Pricing
Immunogen Preparation
•Recombinant protein expression
•Peptide synthesis and conjugation
4 weeks
• Design report
• Protein sample
• SDS-PAGE result
Inquiry
Immunization and Titer test
•5-10 Balb/c immunization Anti-serum titer ELISA test
7-8 weeks
• Anti-serum sample
• ELISA titer
Fusion & Screening
•Myeloma cell culture
•Hybridoma fusion
•Positive clones screening
•2 rounds of subcloning
4weeks
• 10-30 ELISA positive clones
• 1-2 ml supernatant per clone
Antibody production & purification
•hybridoma culture
•Antibody purification
2 weeks
• 3-5 mg antibodies per clone
QC & subtype identification
•SDS-PAGE
•antibody subtype verification
1-2 weeks
• project report

Projects Presentation

Mouse anti-Zebra Fish VASA mAb published in Front Endocrinol (Lausanne). 2022 Feb 8;12:820463

Mouse anti-Zebra Fish VASA mAb published in Front Endocrinol (Lausanne). 2022 Feb 8;12:820463

Mouse anti-P. gingivalis mAb published in ONCOLOGY LETTERS 21: 189, 2021

Mouse anti-P. gingivalis mAb published in ONCOLOGY LETTERS 21: 189, 2021

Rat mAb anti low Density Lipoprotein Receptor published in Circulation. 2022;145:675–687

Rat mAb anti low Density Lipoprotein Receptor published in Circulation. 2022;145:675–687

Mouse anti-SETD3 Mab published in Cell Death Dis. 2019;10(3):183.

Mouse anti-SETD3 Mab published in Cell Death Dis. 2019;10(3):183.