Transient Protein Expression Services in Mammalian Cells
Mammalian cells possess protein folding and post-translational modification functions, and the recombinant proteins expressed by them are the closest to the natural protein structure of higher organisms in terms of molecular structure, physicochemical properties, and biological functions. Elabscience has an experienced technical team that uses high-density suspension-cultured CHO and HEK293 cells as expression hosts. Using independently developed protein expression vectors, it can ensure protein expression levels in a short period of time, with a purity of over 95%. The specific content includes:
• Gene Design and Codon Optimization Expression Evaluation Report
• Selectable eukaryotic expression vectors of 6× His, Fc, Flag, HA, Biotin, etc.
• Transient transfection of HEK293/CHO cells, protein purification conditions, and SDS-PAGE identification results
• Subsequent processing of proteins, such as tag removal, endotoxin removal, protein labeling, MS identification, and other related services
Features of Transient Protein Expression in Mammalian Cells
Well experienced
One-stop service
Experimental success rate ≥ 98%
Independently developed, unique vector
Service Process
| Service Categories | Service Name | Details | Time | Deliverables | Price |
|---|---|---|---|---|---|
| Transient Expression of Mammalian Cellular Proteins | Gene synthesis | Gene synthesis and codon optimization | 1 week |
① A successfully constructed cloning vector ② Sequencing report |
Inquiry |
| Vector construction | Cloning to expression vector | 1 week | |||
| Plasmid construction | |||||
| Large-scale plasmid production | |||||
| Expression evaluation | Transforming suitable cells (HEK293F/CHO) | 2 weeks | ① Expression of evaluation results |
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| Small-scale transfection | |||||
| SDS-PAGE analysis to evaluate protein expression | |||||
| Transiently transfected expression | Transiently transfect cells | 2 weeks |
① Feasibility report ② If expression purification is feasible, provide a certain amount of protein samples to customers based on the expression situation |
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| Cell supernatant affinity purification | |||||
| SDS-PAGE analysis to evaluate protein expression | |||||
| Large-scale expression and purification (optional) | Choice of 1L scale-up culture or bioreactor | Determined by volume |
① Purified protein ② SDS-PAGE report ③ Experimental report |
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| Affinity purification | |||||
| SDS-PAGE analysis to evaluate protein expression |
Service Flow
Customer consultation and request
Scheme determination and contract signing
Project initiation
Project initiation
Protein delivery submit a lab report
Case Presentation
Transiently expressed protein of interest in HEK293 cells
