New Product Launch│One Assay, Two Markers Simplifies Full Glutathione Profiling

Glutathione is a key marker of antioxidant status, and accurately measuring it is essential for oxidative stress research. Introducing Elabscience^® new Total Glutathione (T-GSH) And Reduced Glutathione (GSH) Fluorometric Assay Kit (E-BC-F045), a high-sensitivity tool for precise, single-run detection of T-GSH and GSH. It also enables calculation of Oxidized Glutathione (GSSG) and the GSH/GSSG ratio, delivering reliable data for studies on oxidative stress and cell death mechanisms.

Table of Contents

1. Fluorometric assay principle for T-GSH and GSH detection

2. Technical highlights and experimental utility

3. Performance evaluation and quantitative analysis

4. Validation in cell-based experimental models

5. Related assay kits and supporting reagents

01 Fluorometric assay principle for T-GSH and GSH detection

This kit uses monochlorobimane as a fluorescent probe. Monochlorobimane has low initial fluorescence; its chlorine atom is electrophilic and reacts with the sulfhydryl group in GSH via nucleophilic substitution, forming a stable thioether bond and increasing fluorescence. Adding Glutathione Reductase (GR) reduces GSSG in the sample to GSH, allowing total glutathione measurement.

Fig. 1 Principle of the Total Glutathione (T-GSH) and Reduced Glutathione (GSH) Fluorometric Assay Kit (E-BC-F045).

02 Technical highlights and experimental utility

Multiplex Detection

Simultaneously measure T-GSH and GSH, and calculate GSSG content and GSH/GSSG ratio, providing a comprehensive redox status assessment in one go.

Broad Sample Compatibility

Works with serum, plasma, animal tissues, and cells, adapting to diverse research needs.

Optimized for Cutting-Edge Research

Specifically designed for studies on oxidative stress, ferroptosis, cuproptosis, and disulfidptosis to support your next discovery.

03 Performance evaluation and quantitative analysis

The standard curves show excellent linearity and high sensitivity. 

Fig. 2 GSH Standard Curve.

Fig. 3 GSSG Standard Curve. 

04 Validation in cell-based experimental models

We tested the kit using a Jurkat cell apoptosis model induced by camptothecin. As shown in Fig. 4, intracellular GSH levels dropped significantly in the treated group compared to the untreated control. This confirms the expected oxidative stress response and GSH consumption during camptothecin-induced apoptosis, matching published data and validating the kit's sensitivity and reliabilit

Fig. 4 GSH levels in camptothecin-induced Jurkat cell apoptosis model.

Experimental Protocol

1) Prepare Jurkat cells (1×10⁶ cells/mL in 1640 medium). Seed 1 mL per well in a 24-well plate. Label as Untreated and Induced groups.

2)  Add 2 μL camptothecin (5 mM) to the Induced group wells.

3) Incubate at 37°C, 5% CO₂ for 4 hours, then harvest cells.

05 Related assay kits and supporting reagents

Table 1. Key Elabscience^® Assay Kits for Oxidative Stress and Antioxidant Research