Fluorescence microplate reader
GSH/GSSG
GSH
GSSG
Total Glutathione (T-GSH) And Reduced Glutathione (GSH) Fluorometric Assay Kit (E-BC-F045)
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For research use only.
| Detection Principle |
"Glutathione is a thiol-containing tripeptide compound widely distributed in organisms, participating in various important physiological processes. It protects cells from oxidative damage and detoxifies drug metabolites. Glutathione exists in cells in two forms: reduced glutathione (GSH) and oxidized glutathione (GSSG). GSH can react with the probe to generate a fluorescent compound. The measured fluorescence value is then substituted into a standard curve to calculate the GSH content. After adding a reducing reagent, GSSG is reduced to GSH, thereby determining the total glutathione content in the sample." |
| Synonyms | GSH;T-GSH |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=390 nm/490 nm) |
| Research Area | Oxidative Stress , Ferroptosis , Cuproptosis , Disulfidptosis |
| Other Reagents Required | Normal saline (0.9% NaCl)、3 mol/L perchloric acid、3 mol/L NaOH、double distilled water、PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 9.16 μmol/L |
| Detection Range | 9.16-1000 μmol/L |
| Precision | inter-assay CV: 5.0%-5.9% | intra-assay CV: 2.6%-5.0% |
| Sample Volume | 10 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 10% Mouse heart tissue homogenization | 1 |
| 10% Mouse muscle tissue homogenization | 1 |
| 1×10^6 RAW264.7 cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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